|Number of samples:||21|
|Release date:||Oct 1 2016|
|Last update date:||Oct 24 2018|
|Genes:||FBXL19, RNF20, HIST2H2BE, MGST2|
|Dataset link||Fbxl19 recruits Rnf20 to CpG Islands and promotes H2B mono-ubiquitination [ChIP-Seq]|
ChIP sequenicngs were performed for Fbxl19 and H2Bub1 in mouse ES cells. To perform Fbxl19 ChIP-seq we generated Fbxl19-overexpressing flag-biotin tagged clones and selected overexpression (OE)-high (more than 5 fold) and OE-low (sub-endogeous level) clones. Using these two OE-high and OE-low clones, we performed ChIP-seq of Fbxl19 using Streptavidin-conjugated beads. We also conducted H2Bub1 ChIP-seq under various conditions: normal, differentiation, Fbxl19-OE, Fbxl19-KD, and Fbxl19-KD under differentaiton conditions. Input was also sequenced and used for control.
Citations per year
Nucleic Acids Res