Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 21
Release date: Oct 1 2016
Last update date: Oct 24 2018
Access: Public
Genes: FBXL19, RNF20, HIST2H2BE, MGST2
Taxon: Mus musculus
Dataset link Fbxl19 recruits Rnf20 to CpG Islands and promotes H2B mono-ubiquitination [ChIP-Seq]

Experimental Protocol


ChIP sequenicngs were performed for Fbxl19 and H2Bub1 in mouse ES cells. To perform Fbxl19 ChIP-seq we generated Fbxl19-overexpressing flag-biotin tagged clones and selected overexpression (OE)-high (more than 5 fold) and OE-low (sub-endogeous level) clones. Using these two OE-high and OE-low clones, we performed ChIP-seq of Fbxl19 using Streptavidin-conjugated beads. We also conducted H2Bub1 ChIP-seq under various conditions: normal, differentiation, Fbxl19-OE, Fbxl19-KD, and Fbxl19-KD under differentaiton conditions. Input was also sequenced and used for control.

Repositories


GEO

GSE76569

ENA

SRP068136

BioProject

PRJNA308074

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Contact


Bum-Kyu Lee

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