Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Aug 22 2009
Last update date: May 11 2017
Access: Public
Chemicals: Ethanol, Cocaine, Morphine
Dataset link Selective boosting of transcriptional and behavioral responses to drugs of abuse by histone deacetylase inhibition

Experimental Protocol


Striatal tissue was dissected from two months-old Swiss-Albino male mice subjected to behavioral sensitization. Behavioural sensitization: Locomotor sensitization induced by morphine was evaluated using a protocol divided into two phases: induction and challenge. The induction phase involved six trials on alternate days, one trial per day. On each one of these trials, mice received and injection of saline or sodium butyrate (300 mg/kg) immediatedly followed by a second injection of morphine (20 mg/kg). The challenge phases consisted of a single trial conducted 7 days after the last test of the induction phase. In this case, all animals received a single injection of morphine (20 mg/kg). Striatal RNA was extracted 1h after the morphine challenge in groups of four animals that received either saline (N=6) or morphine (N=3), or the sodium butyrate-morphine (N=3) co-treatment during the sensitization induction phase.

Repositories


GEO

GSE17731

ArrayExpress

E-GEOD-17731

BioProject

PRJNA118381

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Contact


jose lopez
jose p lopez