Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 10
Release date: Nov 21 2016
Last update date: Jul 10 2017
Access: Public
Diseases: Deficiency Diseases
Chemicals: 5-Methylcytosine
Dataset link Tet-dependent transcriptional programs during mESC differentiation

Experimental Protocol


Total RNA obtained from ES cells under the following conditions: (i) WT and Tet3 KO mESCs differentiating into neuroectoderm under SFEB culture (days 6 and 10); and (ii) Control and Tet3-overexpressing mESCs in non-permissive neural differentiation conditions containing FBS (days 4 and 7). Multiplexed libraries were constructed using SOLiD Total RNA-seq kit. Libraries were sequenced on the SOLiD 5500 platforms according to the manufacturer's instructions.

Repositories


GEO

GSE89314

ENA

SRP092292

BioProject

PRJNA351756

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Contact


ANJANA RAO