Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Apr 9 2009
Last update date: Aug 28 2018
Access: Public
Diseases: Leukemia, Leukemia, Myeloid, Acute
Dataset link Linkage of Meis1 leukemogenic activity to multiple downstream effectors including Trib2 and Ccl3

Experimental Protocol


Establishment of pre-leukemic bone marrow cell lines following transduction with ND13 have been previously described (Pineault, Abramovich et al. 2005). In brief, lines were established from BM cells previously treated with 5-fluorouracil (5-FU) from (C57Bl/6Ly-Pep3b x C3H/HeJ) F1 (PepC3) (Ly5.1+/Ly5.2+) mice freshly transduced with the ND13-GFP or ND13pac virus and maintained in liquid culture. To generate ND13+Meis1 or ND13+Meis1 mutant BM cell lines, the ND13 BM cells were transduced by co-cultivation on irradiated (4,000 cGy) E86 producers for Meis1-YFP (or Meis1 mutant forms), respectively, for a period of 2 days in the presence of 5 µg/ml of protamine sulfate. Three independent experiments were performed for each of the four different conditions included in the study.

Repositories


GEO

GSE15541

ArrayExpress

E-GEOD-15541

BioProject

PRJNA115973

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Contact


Lars Palmqvist