Computational protocol: DNA Barcode ITS Effectively Distinguishes the Medicinal Plant Boerhavia diffusa from Its Adulterants

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Protocol publication

[…] The DNA sequences were compared and aligned using the programs ClustalW and MULTALIGN (http://www.multalin.toulouse.inra.fr/multalin/). Further, the DNA sequences were subjected to BLAST (http://www.ncbi.nlm.nih.gov/blast/blast.cgi) for better identification of sequence at species level. Phylogenetic trees were constructed with the Minimum Evolution method using MEGA 4.0. The intra-specific variation between the species was calculated using MEGA 4.0 and StatsDirect was used to calculate the Wilcoxon signed rank . […]

Pipeline specifications

Software tools Clustal W, MultAlin, MEGA
Application Phylogenetics