|Application:||Gene expression microarray analysis|
|Number of samples:||11|
|Release date:||Aug 15 2014|
|Last update date:||Mar 6 2018|
|Diseases:||T-Lymphocytopenia, Idiopathic CD4-Positive|
|Dataset link||Gene Expression in Effector T cells, wildtype of Thpok-deficient|
We activated naïve CD4 T cells (either wild-type or Thpok-deficient) and CD8 T cells (wild-type) in vitro under Th1 conditions. Differentiated effectors were sorted 4 days after activation into CD4+CD8- and CD4-CD8+ (wild-type) and CD4+CD8- and CD4+CD8+ (Thpok-deficient) subsets. Total RNA was extracted from sorted subsets and processed for microarray analyses (Affymetrix Mouse Exon 1.0 ST array) at the NCI microarray facility, following the manufacturer’s recommendation. Data is from 3 replicates (except wild-type CD4-CD8+ cells, for which two samples only were processed), generated from two distinct cell preparations.
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