Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 11
Release date: Aug 15 2014
Last update date: Mar 6 2018
Access: Public
Diseases: T-Lymphocytopenia, Idiopathic CD4-Positive
Dataset link Gene Expression in Effector T cells, wildtype of Thpok-deficient

Experimental Protocol


We activated naïve CD4 T cells (either wild-type or Thpok-deficient) and CD8 T cells (wild-type) in vitro under Th1 conditions. Differentiated effectors were sorted 4 days after activation into CD4+CD8- and CD4-CD8+ (wild-type) and CD4+CD8- and CD4+CD8+ (Thpok-deficient) subsets. Total RNA was extracted from sorted subsets and processed for microarray analyses (Affymetrix Mouse Exon 1.0 ST array) at the NCI microarray facility, following the manufacturer’s recommendation. Data is from 3 replicates (except wild-type CD4-CD8+ cells, for which two samples only were processed), generated from two distinct cell preparations.

Repositories


GEO

GSE57846

ArrayExpress

E-GEOD-57846

BioProject

PRJNA248219

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Contact


David Salomon

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