Computational protocol: Subtle reproductive impairment through nitric oxide-mediated mechanisms in sea urchins from an area affected by harmful algal blooms

Similar protocols

Protocol publication

[…] For protein identification, sea urchins ovaries were collected and the protein extracts were digested by trypsin sequence grade upon precipitation in 10% TCA (trichloroacetic acid), reduction with 45 mM dithiothreitol and alkylation with 100 mM iodoacetamide. MS/MS analysis was carried out by a LTQ-Orbitrap Velos (Thermo Fisher Scientific), as previously described. Data Base searching was performed using the Sequest search engine of Proteome Discoverer 1.1 (Thermo Fisher Scientific). […]

Pipeline specifications

Software tools Comet, Proteome Discoverer
Application MS-based untargeted proteomics
Organisms Paracentrotus lividus
Diseases Abnormalities, Drug-Induced, Bloom Syndrome
Chemicals Nitric Oxide