Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 77
Release date: Oct 31 2017
Last update date: Oct 29 2018
Access: Public
Dataset link Differential Expression of MicroRNAs in Leprosy Skin Lesions

Experimental Protocol


The sequence of events for this study followed the order below. Patients who were treated for leprosy were examined by leprologists and submitted to two biopsy procedures. One biopsy was processed for histopathological analysis and bacilloscopy, the other was immediately stored in RNAlater® solution (Ambion) for further RNA extraction. Sixty-eight samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) were selected for analysis. In addition, nine skin biopsies from healthy individuals were usedas controls (CC). Differentially expressed miRNAs identified in the cDNA microarray assay were validated by quantitative RT-PCR.

Repositories


GEO

GSE102314

BioProject

PRJNA397418

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Contact


Alex Carvalho

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