Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Aug 9 2011
Last update date: Mar 23 2012
Access: Public
Chemicals: Nitrogen
Dataset link Transcriptional Responses of Karenia brevis to Nitrogen Depletion and Addition

Experimental Protocol


Cultures were acclimated to 10 µM nitrate for a minimum of two months during which time cultures were transferred weekly in log phase. For addition experiments, nutrient replete and N-limited 1L cultures were grown to stationary phase. (Day 9) Using sodium nitrate, 155 µM NO3 was added to stationary phase cultures. Nutrient replete cultures and N-limited cultures were harvested at the time of nutrient addition (Time 0; n=3) and total RNA extracted. Following nitrogen addition, triplicate cultures were harvested at 4, 12, 24, and 48 h post-addition and total RNA extracted. One color arrays were then run on all biological replicates (n=3 at each time point).

Repositories


GEO

GSE28362

BioProject

PRJNA139423

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Contact


Frances Van Dolah