|Application:||Gene expression microarray analysis|
|Number of samples:||18|
|Release date:||Aug 9 2011|
|Last update date:||Mar 23 2012|
|Dataset link||Transcriptional Responses of Karenia brevis to Nitrogen Depletion and Addition|
Cultures were acclimated to 10 µM nitrate for a minimum of two months during which time cultures were transferred weekly in log phase. For addition experiments, nutrient replete and N-limited 1L cultures were grown to stationary phase. (Day 9) Using sodium nitrate, 155 µM NO3 was added to stationary phase cultures. Nutrient replete cultures and N-limited cultures were harvested at the time of nutrient addition (Time 0; n=3) and total RNA extracted. Following nitrogen addition, triplicate cultures were harvested at 4, 12, 24, and 48 h post-addition and total RNA extracted. One color arrays were then run on all biological replicates (n=3 at each time point).
Frances Van Dolah