Computational protocol: Neonatal AAV delivery of alpha-synuclein induces pathology in the adult mouse brain

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Protocol publication

[…] Brightfield images were captured using the Aperio slide scanner (Vista, CA, USA). Fluorescent images were taken with a 40 x Plan-Apochromat objective using a Zeiss AxioObserver equipped with an ApoTome Imaging System (Zeiss). Microglial and astroglial cell counts and morpholological analysis (process length and cell body size) were quantified using MetaMorph Image Analysis Software (Molecular Devices) with the neurite outgrowth application module []. MetaMorph Software with the cell counting module was used to assess the burden of NeuN positive neurons. First, ImageScope® software (v12.1; Leica Biosystems) was used to annotate the cortex on mid-sagittal sections stained for NeuN for each mouse. Then, Positive Pixel Count Algorithm was established to recognize and quantify NeuN positive cells .The output parameter was the number of NeuN-positive neurons per given mm2 area annotated. […]

Pipeline specifications

Software tools MetaMorph, ImageScope
Application Whole slide imaging
Organisms Adeno-associated virus, Mus musculus, Homo sapiens, Rattus norvegicus
Diseases Dementia, Parkinson Disease, Lewy Body Disease