|Number of samples:||45|
|Release date:||Jan 4 2018|
|Last update date:||Nov 27 2018|
|Diseases:||Brain Neoplasms, Glioma, Neoplasms|
|Computational protocol:||IPA, VENNY, starBase|
|Dataset link||Full-coverage landscape of extracellular RNAs, coding and non-coding, secreted by human glioma stem cells|
Four low-passage patient-derived glioblastoma stem cell cultures were grown as neurospheres in serum-free media. Microvesicles (MVs), exosomes, and ribonucleoproteins (RNPs) were separated from conditioned media using sequential filtration, and RNA was isolated from each fraction. Cellular RNA was isolated from cultures in parallel. RNA was also isolated from MVs, exosomes, and RNPs from fresh media. Both long RNA library and small RNA libraries were prepared for each RNA sample, and sequenced on HiSeq2000. Cellular RNA of human or mouse primary normal brain cells (neurons, astrocytes, microglia, and endothelial cells) were also sequenced for small RNA libraries.
DOI: 10.1038/s41467-017-01196-xcall_split See protocol