Dataset features

Specifications


Application: sRNA-seq analysis
Number of samples: 45
Release date: Jan 4 2018
Last update date: Nov 27 2018
Access: Public
Diseases: Brain Neoplasms, Glioma, Neoplasms
Computational protocol: IPA, VENNY, starBase
Dataset link Full-coverage landscape of extracellular RNAs, coding and non-coding, secreted by human glioma stem cells

Experimental Protocol


Four low-passage patient-derived glioblastoma stem cell cultures were grown as neurospheres in serum-free media. Microvesicles (MVs), exosomes, and ribonucleoproteins (RNPs) were separated from conditioned media using sequential filtration, and RNA was isolated from each fraction. Cellular RNA was isolated from cultures in parallel. RNA was also isolated from MVs, exosomes, and RNPs from fresh media. Both long RNA library and small RNA libraries were prepared for each RNA sample, and sequenced on HiSeq2000. Cellular RNA of human or mouse primary normal brain cells (neurons, astrocytes, microglia, and endothelial cells) were also sequenced for small RNA libraries.

Repositories


GEO

GSE93143

ENA

SRP096035

BioProject

PRJNA360129

Download


Dataset Statistics

info

Citations per year

Dataset publication