Computational protocol: Angiotensin type 1 receptor antagonist losartan, reduces MPTP-induced degeneration of dopaminergic neurons in substantia nigra

Similar protocols

Protocol publication

[…] Atgr2, TH and GAPDH mRNA expression profiles were done by quantitative real-time PCR on TH+ neurons from the SNpc or VTA. Primers used are listed in table . The primers (18–22 mer) were designed using Primer3 []. These primer sets were designed to amplify small amplicons for candidate mRNAs ranging from 100–300 bp in size. First-strand cDNA synthesis was carried out on mRNA extracted with SuperscriptTM first-strand synthesis kit (Invitrogen Carlsbad, CA) according to the manufacturer's specifications. Real-time RT-PCR was carried out in a 96 well plate using a MyiQ iCycler (BioRad, Hercules, CA), and SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA). A concentration curve with known concentrations of whole brain cDNA extracts from 12-week-old male C57BL/6 mice was used to calculate standard curves and quantitate the products. The final concentration of each transcript was calculated using the MyiQ2.0 software provided by BioRad. [...] Data were analyzed using Statistica software. Statistical significance was determined by one-way ANOVA followed by post-hoc Newman-Keuls multiple comparison tests. Differences in mean values were considered significant at p ≤ 0.05. All data were obtained from at least three independent experiments and are represented as a mean ± SEM. […]

Pipeline specifications

Software tools Primer3, Statistica
Applications Miscellaneous, qPCR
Organisms Chrysanthemum x morifolium, Mus musculus
Diseases Parkinson Disease, Neurodegenerative Diseases, Stroke, Drug-Related Side Effects and Adverse Reactions
Chemicals 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Losartan