Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Nov 29 2011
Last update date: Mar 22 2012
Access: Public
Diseases: Urinary Tract Infections
Chemicals: Amino Acids, Ammonia, Carbon, Iron, Nitrogen, Glutamic Acid, Pyruvic Acid
Dataset link Transcriptome of Proteus mirabilis in the murine urinary tract

Experimental Protocol


Voided urine from female CBA/J mice infected with Proteus mirabilis was collected and pooled in RNA stabilizing reagent (RNAprotect). Urine was collected at 1, 3, and 7 d postinfection. RNA was isolated from urine and log-phase LB cultures, converted to cDNA, and labeled with CyDye. Three arrays were completed per time point (9 arrays total). Slides were scanned with a ScanArray Express microarray scanner (Perkin Elmer) at 10 μm resolution and quantified using ScanArray Express software. Resulting data were normalized by total intensity and median spot intensities were identified using MIDAS (v. 2.22) software.

Repositories


GEO

GSE25977

ArrayExpress

E-GEOD-25977

BioProject

PRJNA135519

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Contact


Melanie Pearson
Melanie M Pearson