Computational protocol: Cotton Ascorbate Oxidase Promotes Cell Growth in Cultured Tobacco Bright Yellow-2 Cells through Generation of Apoplast Oxidation

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Protocol publication

[…] Protein sequences were aligned using the ClustalX program version 2.1 (European Bioinformatics Institute, Cambridge, UK) with default settings. Online software of conserved domains (CD) at the National Center for Biotechnology Information (NCBI) website (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi) was used for functional motif analysis, and online software packages of SignalP and TMpred from the ExPASy website (http://www.expasy.org/) were used for analyses of signal peptide and transmembrane regions. […]

Pipeline specifications

Software tools Clustal W, SignalP, TMpred
Databases ExPASy
Application Membrane protein analysis
Organisms Gossypium hirsutum, Nicotiana tabacum, Allium cepa
Diseases Machado-Joseph Disease