Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Aug 1 2007
Last update date: Aug 28 2018
Access: Public
Dataset link Effects of JNK on TGF-beta responses of mouse B lymphoma derived A20 cells

Experimental Protocol


The following six samples were prepared: untreated A20 cells (non-stimulated, DMSO): A20 cells cultured with SP600125 for 24 h (non-stimulated, SP600125): A20 cells stimulated with TGF-beta for 12 h (TGF-beta 12 h, DMSO) and 24 h (TGF-beta 24 h, DMSO): and A20 cells stimulated with TGF-beta in the presence of SP600125 for 12 h (TGF-beta 12 h, SP600125) and 24 h (TGF-beta 24 h, SP600125), respectively. Total RNA was prepared and hybridized to the Affymetrix Mouse Genome 430 2.0 array. Genes whose expression was increased by more than 2-fold at either 12 or 24 h after TGF-beta stimulation were identified as TGF-beta inducible genes. Amongst them, we identified genes whose induction levels were reduced by more than 75% by co-treatment with the JNK inhibitor SP600125.

Repositories


GEO

GSE7503

ArrayExpress

E-GEOD-7503

BioProject

PRJNA100489

Download


Contact


shin yonehara