Computational protocol: Surface Area of Patellar Facets: Inferential Statistics in the Iraqi Population

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Protocol publication

[…] Materials used included 40 human patellae of the Iraqi ethnicity; both age and gender were unknown. The patellae included 20 right patellae and 20 left patellae. These patellae belonged to deceased members of the Iraqi ethnicity. The measured morphometric parameters for each patella included two patellar heights measurement (H1, H2), the maximal width (W) measured perpendicularly to the midpoint of the main facetal vertical ridge (), and the maximal thickness (T). The maximal thickness was measured at the level of the midpoint of the facetal ridge. The surface area of each of the two (lateral and medial) main patellar facets was measured using digital imaging analysis software known as ImageJ software []; these area measurements were accurate down to a thousandth of a square centimetre (cm2). Additionally, the weight of each patella was measured using an electronic balance; these measurements were also accurate to the nearest thousandth of a gramme (gm).Measurements were taken at the Anatomical Specimens' Unit at the Department of Anatomy and Cellular Biology. Further, to prevent human-made errors and biases while measuring, two of the authors of this paper recorded each morphometric parameter twice. Linear measurements (H1, H2, W, and T) were done using a standard digital electronic Vernier Caliper (Vernier micrometre gauge tool, UPC number 814870023454), and the unit of tabulated data () is the millimetre (mm). When the two measurements were different to the nearest 1/10th of a millimetre, a 3rd independent reading was taken to resolve the numerical disparity. This step was followed by taking the average numerical value for each of the measured morphometric parameters (). Statistical analyses, descriptive () and inferential, were done by using the Statistical Package for Social Sciences (SPSS 22) and Microsoft Excel 2016. Inferential statistics included the application of analysis of variance (ANOVA), linear regression models, and Student's t-test for paired and unpaired data. Statistical inference, at an alpha value (α) of 0.05, was considered to test the level of significance.A systematic review of the literature was done from 2 August 2016 to the 18 November 2016, to collect the most valid and up-to-date literature on the topic. The literature review was done across medical databases: PubMed, the Cochrane Library, Scopus, Google Scholar, and unpublished literature (grey literature). Prespecified keywords were used, in combination with Boolean operators []. The total number of papers pertinent to the study topic, after exclusion of duplicate articles, was 37 papers. These papers were scanned and filtered, after a detailed inspection of each paper's title, abstract, and the main body of the manuscript. The papers were also assessed for their level of evidence using critical appraisal tools []. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Homo sapiens