Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 43
Release date: Oct 4 2008
Last update date: Mar 19 2012
Access: Public
Diseases: Hyperlipoproteinemia Type II, Myositis, Neoplasms, Psoriasis, Corneal Neovascularization
Dataset link Quantification of vascular lineage-specific differentiation, psoriasis (chronic inflammation) study

Experimental Protocol


Guided by the gene array results, we selected 54 LEC-specific genes and 31 BEC-specific genes, based upon their consistent and strong specific expression in LEC or BEC, as well as on their assignment to important biological pathways. In addition, the five pan-endothelial cell marker genes PECAM-1, vWF, KDR, TEK, CDH5 and the six endogenous control genes ACTB, GAPDH, PGK1, PPIA, RPLP0 and S18 were included in the design of the LD-MDA. After extraction of total RNA, the mRNA expression levels of the 96 genes were analyzed by quantitative RT-PCR using the 7900HT Real-Time PCR System (Applied Biosystems).

Repositories


GEO

GSE11307

ArrayExpress

E-GEOD-11307

BioProject

PRJNA109199

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Contact


Jay Shin
Jay Woo Shin