|Application:||Gene expression microarray analysis|
|Number of samples:||43|
|Release date:||Oct 4 2008|
|Last update date:||Mar 19 2012|
|Diseases:||Hyperlipoproteinemia Type II, Myositis, Neoplasms, Psoriasis, Corneal Neovascularization|
|Dataset link||Quantification of vascular lineage-specific differentiation, psoriasis (chronic inflammation) study|
Guided by the gene array results, we selected 54 LEC-specific genes and 31 BEC-specific genes, based upon their consistent and strong specific expression in LEC or BEC, as well as on their assignment to important biological pathways. In addition, the five pan-endothelial cell marker genes PECAM-1, vWF, KDR, TEK, CDH5 and the six endogenous control genes ACTB, GAPDH, PGK1, PPIA, RPLP0 and S18 were included in the design of the LD-MDA. After extraction of total RNA, the mRNA expression levels of the 96 genes were analyzed by quantitative RT-PCR using the 7900HT Real-Time PCR System (Applied Biosystems).
Jay Woo Shin