Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Aug 17 2011
Last update date: Jul 31 2017
Access: Public
Genes: Rap1b, FOSB, FOS, EGR2, RAP1B, RAP1A
Taxon: Rattus norvegicus
Dataset link Optimized amplification and single-cell analysis identify GnRH-mediated activation of Rap1b in primary rat gonadotropes.

Experimental Protocol


Primary rat gonadotrope cells were exposed to 10 nM GnRH for 40 min, then harvested and processed for RNA extraction using a Qiagen RNeasy mini kit (Qiagen, Valencia, CA). A total of 12 Affymetrix Rat Expression Array 230 v2.0, namely 6 GnRH-treated and 6 vehicle-treated samples, each containing 31,000 gene clusters, were used. Data analysis was performed by Affymetrix GeneChip Operating System (GCOS). A gene was considered to be up-regulated by GnRH if there is at least 50% concordance across multiple pairwise comparisons of GnRH- vs. vehicle-treated microarrays, and if the fold-change was at least 1.50.

Repositories


GEO

GSE31399

ArrayExpress

E-GEOD-31399

BioProject

PRJNA145721

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Contact


Tony Yuen
Hanna Pincas