Expression analysis of Saccharomyces cerevisiae INVSc1 under accumulation of the bacterial alarmone ppGpp

ppGpp accumulation caused by ectopic expression of RelA in Saccharomyces cerevisiae gave rise to marked changes in gene expression with both upregulation and downregulation, including changes in mitochondrial gene expression. The most prominent upregulation (38-fold) was detected in the function-unknown hypothetical gene YBR072C-A, followed by many other known stress-responsive genes. ppGpp acuumulation resulted in enhancement of tolerance against various stress stimuli, such as osmotic stress, ethanol, hydrogen peroxide, and high temperature.

Dataset features

Information

Specifications

Application:	Gene expression microarray analysis
Number of samples:	2
Release date:	May 31 2012
Last update date:	Sep 4 2012
Access:	Public
Diseases:	Bacterial Infections
Chemicals:	Ethanol, Hydrogen Peroxide
Dataset link	Expression analysis of Saccharomyces cerevisiae INVSc1 under accumulation of the bacterial alarmone ppGpp

Experimental Protocol

A two chip study using total RNA recoverd from the Saccharomyces cerevisiae TN2080 (accumulating ppGpp) and TN2077 (vector control) grown to mid-growth phase (8h) in SC-uracil medium. The plasmid pYC2/CT (V5-epitope tag vector) was used as a vector to express Sj-RSH.

Repositories

GEO

GSE38239

ArrayExpress

E-GEOD-38239

BioProject

PRJNA167742

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Contact

Kozo Ochi