Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 56
Release date: Sep 16 2009
Last update date: Oct 29 2018
Access: Public
Diseases: Colitis, Ulcerative, Irritable Bowel Syndrome
Dataset link Genome-wide gene expression analysis of mucosal colonic biopsies and isolated colonocytes...

Experimental Protocol


Adjacent mucosal colonic biopsies were attained endoscopically from the descending colon in patients with active UC (n=8), quiescent UC (n=9), and in controls (n=10). After extraction of total RNA, genome-wide gene expression data were acquired using Human Genome U133 Plus 2.0 GeneChip Array (Affymetrix, Santa Clara, CA). Amplification was required to obtain sufficient amounts of labelled complementary RNA (cRNA) target for analysis with arrays. Data analysis was carried out by principal component analysis and projection to latent structure-discriminant analysis using the SIMCA-P11 software (Umetrics, Umeå, Sweden).

Repositories


GEO

GSE13367

ArrayExpress

E-GEOD-13367

BioProject

PRJNA109787

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