Computational protocol: Germination Shifts of C3 and C4 Species under Simulated Global Warming Scenario

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Protocol publication

[…] Data were analysed using SPSS (version 13.0, SPSS Inc., Chicago, Illinois, USA). Germination percentage and germination rate of C3 or C4 species were the average of the three species with the same photosynthetic type. The effects of different alternating temperature treatments in each regime and the plant photosynthesis type (C3 and C4) on germination percentage and germination rate were examined using two-way ANOVA. Germination times for 1 percent seed (1% germination) were calculated from the cumulative germination curves in AW and SW temperature regimes for the six species and converted to rates in this study, which represents the initiative germination time of each batch of seeds. This standard (1% germination) was used instead of 50% germination because seed germination of certain species was less than 50% for some temperature treatments (see ). Thermal time model (TT model) parameters were analyzed using repeated probit regression as described previously . The average daily temperatures were used for determining alternating temperatures. The model parameter (T b and θ 1) differences between C3 and C4 species, or between AW and SW temperature regimes within one photosynthetic type, were tested by Paired-Samples T-tests (2-tailed). The relationships between germination percentages/rates and the average temperatures in AW and SW regimes were fitted by either linear or quadratic regressions using SigmaPlot (version 10.0, Systat Software Inc., Richmond, California, USA), with 95% confidence of the fitted lines given. The differences in the slope (a in the equation) of the regression between AW and SW regimes for germination of C4 species were tested using the SMATR package in R software . Pearson correlation analysis was carried out to test the correlation between GP/GR and temperature metrics (minimum, maximum, DTR, average) from the four alternating temperature regimes, totally 29 treatments. […]

Pipeline specifications

Software tools SPSS, SigmaPlot
Application Miscellaneous
Chemicals Carbon