Dataset features

Specifications


Application: sRNA-seq analysis
Number of samples: 24
Release date: Mar 24 2016
Last update date: Jan 26 2018
Access: Public
Taxon: Caenorhabditis elegans
Dataset link A Tunable Mechanism Determines the Transgenerational Duration of Small RNA Inheritance in C.elegans

Experimental Protocol


Examination of dsRNA induced transgenerational inheritance in lineages with and without second trigger enhancment Small-RNA sequencing of P0-F3 generations of [1] worms that were exposed to anti-gfp dsRNA at the P0 generation and transferred to control bacteria ("empty vector") at the next generations (F1-F3) [2] worms that were exposed to anti-gfp dsRNA at the P0 generation, transferred to an additional anti-mCherry RNAi plates at the F1 generation ("2nd trigger") and moved to control bacteria at the F2 generations for two generations (F2-F3) and [3] control group of worms that were grown on "empty-vector" plates and were not exposed to RNAi at any stage.

Repositories


GEO

GSE77654

ENA

SRP069756

BioProject

PRJNA311152

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Contact


Leah Houri-Ze'evi