DAOSTORM protocols

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DAOSTORM specifications

Information


Unique identifier OMICS_06528
Name DAOSTORM
Software type Package/Module
Interface Command line interface, Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

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Publication for DAOSTORM

DAOSTORM in pipeline

2016
PMCID: 4973222
PMID: 27488312
DOI: 10.1038/srep30826

[…] data analysis becomes a challenge because, for most phase-based measurements, the psf cannot be simply approximated and fit by simple equations like the 2d gaussian function. algorithms such as 3d daostorm can analyze astigmatism-based 3d images. 3d falcon can be used to analyze astigmatic images, biplane images, as well as hybrid images that combine astigmatic and biplane methods. however, […]


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DAOSTORM in publications

 (20)
PMCID: 5913229
PMID: 29686251
DOI: 10.1038/s41598-018-24033-7

[…] faster cameras, and those that allow a higher density of emitted fluorophores in each individual frame by discerning the overlapping single-molecule images with computational algorithms, such as daostorm,, bayesian statistics and compressed sensing,. for sted or sim, the improvement in temporal resolution has been focused on the parallelization of multiple patterns in one camera exposure […]

PMCID: 5859009
PMID: 29555914
DOI: 10.1038/s41598-018-22297-7

[…] were collected scanning of a 5 µm-depth volume at a step size of 1 µm and 100 fovs were collected for each smfish probe set. smfish spots were detected using the multi-emitter fitting routine 3d-daostorm for each z section and spots appearing in adjacent z sections at the same x,y location were combined into one. ~1,000 cells per gene were measured in each smfish measurement., we performed […]

PMCID: 5738400
PMID: 29263350
DOI: 10.1038/s41598-017-18303-z

[…] stellar fields, methods that fit populations of single molecules with multiple psfs instead of just one can improve accuracy when detecting overlapping molecules. holden et al. implemented this in daostorm. zhu et al. reported a sparse recovery technique using compressed sensing and showed this could work with much higher molecule densities when compared to daostorm. in summary, single […]

PMCID: 5493635
PMID: 28667336
DOI: 10.1038/s41598-017-04544-5

[…] }_{\max ,b}/{\tau }_{\max ,d}=1$$\end{document}τmax,b/τmax,d=1. while such states are undesirable for localization microscopy unless multiple emitter localization techniques such as daostorm are employed, methods that use fluctuations only can still be conveniently applied to these techniques., we note that the above example considered the simplified photon emission model […]

PMCID: 5428856
PMID: 28373678
DOI: 10.1038/s41598-017-00622-w

[…] used in the daophot astronomy package to provide higher order corrections to a 2d gaussian for the purpose of fitting the locations and magnitudes of stars, or single molecules, the latter using the daostorm algorithm, the adaptation of daophot for single molecule localization microscopy. b-splines, of which cubic splines are a sub-set, were used previously in localization microscopy to estimate […]


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