Data analysis | Fluorescence resonance energy transfert
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. […] Applications extending from a single molecule to live cells will benefit from multidimensional microscopy techniques, particularly those adapted for optical sectioning and incorporating new algorithms for resolving the component contributions to images of complex molecular systems.
Provides information about relative orientations and favorable dyes conformations. Rotamer Library is a method for potentially replacing molecular dynamics (MD) simulations in Förster resonance energy transfer FRET data interpretation. The method was adapted from the analysis of electron paramagnetic studies and utilizes a library of rotamers describing probe conformations.
Allows users to calculate and display fluorescence resonance energy transfer (FRET) directly on images. PixFRET permits computation by performing a pixel-by-pixel analysis of images and assisting in the determination of spectral bleed-throughs (SBTs). It also assists in the determination of SBT parameters and the display of normalized FRET images.
Represents an integrated solution for data analysis and acquisition. SymPhoTime 64 permits users to target principally the results rather than the data processing. The interface of this tool simplifies the utilization by the user for an individual analysis or measurement process. Different data acquisition modes including interleaved trains of excitation and stimulated emission depletion (STED) laser pulses are integrated in a graphical user interface (GUI).
Offers an application dedicated to single-molecule fluorescence resonance energy transfer (SmFRET) with total-internal-reflection fluorescence (TIRF)-microscopy data analyses. iSMS provides features including molecule localization, detection of bleaching, FRET distribution as well as molecule subpopulation analysis. Additionally, it also proposes functions for generating figures for further publishing and utilities for visualization of the performed data.
Enables platform-independent analysis of Fluorescence resonance energy transfer (FRET) by acceptor photo-bleaching. FRETcalc implements the FRETTH algorithm, that is based on pixel selection and pixel-to-pixel analysis of the experimental images. The software is applicable for FRET estimation in organelles with non-continuous and continuous shapes, as well as for non-protein fluorophores. The output options include histograms and plots of the donor, acceptor, and FRET intensities in the analyzed region of interests (ROIs).
Manages instrument control, image processing and data analysis. SlideBook can drive hundreds of devices including microscopes, stages, lasers, wheels, piezos, scanners or shutters. It acquires data in 3D format over time, color, and specimen locations in customizable experiment protocols. This tool offers a solution to investigate images and obtain statistical data via a wide variety of algorithms while maintaining original data integrity.
Allows calculation of fluorescence resonance energy transfer (FRET) efficiencies pixel-by-pixel in microscopic images. AccPbFRET is an ImageJ plugin that enables users to correct for bleaching of the donor, for the cross-talk of the acceptor and its photoproduct to the donor channel and for partial acceptor photobleaching. The software also provides automatic registration and semi-automatic analysis of large image sets.