Data analysis | Fluorescence resonance energy transfert
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. […] Applications extending from a single molecule to live cells will benefit from multidimensional microscopy techniques, particularly those adapted for optical sectioning and incorporating new algorithms for resolving the component contributions to images of complex molecular systems.
Provides information about relative orientations and favorable dyes conformations. Rotamer Library is a method for potentially replacing molecular dynamics (MD) simulations in Förster resonance energy transfer FRET data interpretation. The method was adapted from the analysis of electron paramagnetic studies and utilizes a library of rotamers describing probe conformations.
Represents an integrated solution for data analysis and acquisition. SymPhoTime 64 permits users to target principally the results rather than the data processing. The interface of this tool simplifies the utilization by the user for an individual analysis or measurement process. Different data acquisition modes including interleaved trains of excitation and stimulated emission depletion (STED) laser pulses are integrated in a graphical user interface (GUI).
Assists users to perform fluorescence decay analysis. DecayFit is a program that includes several features allowing researchers to execute several works: analyze time-resolved fluorescence decays by iterative reconvolution or modified tail fitting; global optimization: analysis of multiple decays simultaneously; time-resolved forster resonance energy transfer (FRET) analysis; or parameter confidence interval estimation.
Enables platform-independent analysis of Fluorescence resonance energy transfer (FRET) by acceptor photo-bleaching. FRETcalc implements the FRETTH algorithm, that is based on pixel selection and pixel-to-pixel analysis of the experimental images. The software is applicable for FRET estimation in organelles with non-continuous and continuous shapes, as well as for non-protein fluorophores. The output options include histograms and plots of the donor, acceptor, and FRET intensities in the analyzed region of interests (ROIs).
Allows users to perform simulation and analysis of forster resonance energy transfer (FRET) in nucleic acids. FRETmatrix is based on a matrix base-centred calculation scheme to build nucleic acid geometries. Moreover, this program can be used, in combination with base–base FRET, for reconstructing the 3D structure of local kinks, such as protein/ligand binding sites, bulges, junctions and DNA lesions.
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