A proteogenomic pipeline that delineates true in vivo proteoforms and generates a protein sequence search space for peptide to MS/MS matching. PROTEOFORMER can be combined with canonical protein databases or used independently for identification of novel translation products. The pipeline makes use of the recently developed next generation sequencing strategy termed ribosome profiling (RIBO-seq) that provides genome-wide information on protein synthesis in vivo.
Discover our proposed protocols. They are easy to use or edit to meet your needs.