Expression data from synchronized L4 C. elegans during starvation

The endoplasmic reticulum (ER) is an organelle associated with lipid metabolism. However, the involvement of the ER in nutritional status-dependent energy homeostasis is largely unknown. The results of this study demonstrate that IRE-1, an ER protein known to be involved in the unfolded protein response, and HSP-4, an ER chaperone, regulate expression of the novel fasting-induced lipases FIL-1 and FIL-2, which induce fat granule hydrolysis upon fasting in C. elegans. RNAi and ectopic expression experiments demostrated that FIL-1 and FIL-2 are both necessary and sufficient for fasting-induced fat granule breakdown. Failure of ire-1 and hsp-4 mutant animals to hydrolyze fat granules during starvation impaired their motility, which was rescued by glucose supplementation of their media, implicating the importance of ire-1/hsp-4-dependent lipolysis for energy supply from stored fat during fasting. Taken together, these data suggest that the ER-resident proteins IRE-1 and HSP-4 are key nutritional sensors that modulate expression of inducible lipases to maintain whole-body energy homeostasis in C. elegans.

Dataset features

Information

Specifications

Application:	Gene expression microarray analysis
Number of samples:	1
Release date:	Apr 15 2009
Last update date:	Mar 21 2012
Access:	Public
Diseases:	Neoplasms, Second Primary
Chemicals:	Glucose
Dataset link	Expression data from synchronized L4 C. elegans during starvation

Experimental Protocol

Synchronized L4 worms were divided into well-fed and 6 hours fasted samples for RNA extraction and hybridization on an Agilent microarray.

Repositories

GEO

GSE15656

ArrayExpress

E-GEOD-15656

BioProject

PRJNA116677

Dataset features

Specifications

Experimental Protocol

Repositories

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