|Application:||Gene expression microarray analysis|
|Number of samples:||11|
|Release date:||Jun 20 2008|
|Last update date:||Aug 28 2018|
|Dataset link||Brassinazole treatment of arf2 and wild-type dark-grown seedlings|
Total RNA was extracted from 4-day-old, etiolated Arabidopsis seedlings grown on 0.5 µM BRZ or mock treatments and used to probe ATH1 microarrays (Affymetrix), according to manufacturer’s protocols. There are three independent biological replicates for each genotype and treatment, except for arf2 mutants with BRZ where only two arrays passed quality control. We performed standard Affymetrix quality-control procedures using the BioConductor packages simpleaffy. Expression was normalized and estimated using the gcRMA package of BioConductor.