Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 11
Release date: Jun 20 2008
Last update date: Aug 28 2018
Access: Public
Dataset link Brassinazole treatment of arf2 and wild-type dark-grown seedlings

Experimental Protocol


Total RNA was extracted from 4-day-old, etiolated Arabidopsis seedlings grown on 0.5 µM BRZ or mock treatments and used to probe ATH1 microarrays (Affymetrix), according to manufacturer’s protocols. There are three independent biological replicates for each genotype and treatment, except for arf2 mutants with BRZ where only two arrays passed quality control. We performed standard Affymetrix quality-control procedures using the BioConductor packages simpleaffy. Expression was normalized and estimated using the gcRMA package of BioConductor.

Repositories


GEO

GSE11216

ArrayExpress

E-GEOD-11216

BioProject

PRJNA106821

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Contact


Jennifer Nemhauser