|Application:||Gene expression microarray analysis|
|Number of samples:||23|
|Release date:||Jan 16 2007|
|Last update date:||Feb 18 2018|
|Diseases:||Neoplasms, Germ Cell and Embryonal|
|Dataset link||Expression data from spec. transcriptional activity of primary mouse embryonic fibroblasts (MEF) in response to serum.|
The gene expression and regulation program at the RNA level early (2h) in response to serum was studied. Total RNA, amplified total RNA and specifically enriched newly transcribed RNA was isolated from serum starved and 2h serum treated MEF cells. This experiment allowed detailed regulatory mechanisms of mRNA expression and the relative contributions of RNA synthesis and turnover within distinct pathways, and identification of genes expressed at low abundance at the transcriptional level of MEF cells early in response to serum.