Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 12
Release date: Oct 28 2015
Last update date: Nov 14 2017
Access: Public
Diseases: Neoplasms
Dataset link A MYC-driven change in mitochondrial dynamics limits stem cell properties of mammary epithelial cells (RNA-seq)

Experimental Protocol


RNA-Seq Experiments in 2 different primary breast epithelial cell lines (HMLE, which were sorted according to CD44/CD24 surface markers & unsorted IMEC). Both cell lines expressed a doxycycline-inducible version of MYC. For the HMLE cell line DGE analysis was performed for the uninduced (EtOH) situation, comparing CD44high vs CD44 low and for the induced situation Dox vs. EtOH for the CD44high population. For the IMEC cell line DGE was performed by comparing Dox-treated populations expressing either Dox-inducible MYC or a vector control which allows to filter out potential effects due to doxycycline treatment.

Repositories


GEO

GSE66250

ArrayExpress

E-GEOD-66250

ENA

SRP055475

BioProject

PRJNA276279

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