Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 12
Release date: Jun 22 2015
Last update date: Oct 10 2018
Access: Public
Chemicals: Cytosine
Computational protocol: Trim Galore!, BSMAP, methylKit, GEO
Dataset link DNA methylation analysis by RRBS in germ cells of mice exposed to Vinclozolin

Experimental Protocol


Pregnant F0 female mice were exposed to two doses (a low dose VD1=1 mg/kg bw/d and a high dose VD2=100mg/kg bw/d) of Vinclozolin in the drinking water during pregnancy. We isolated primordial germ cells at E13.5 and mature sperm from adult F1 males obtained from control and exposed mothers. For PGCs, we sequenced RRBS libraries prepared from one pool isolated from F1 embryos exposed to the low dose, one pool isolated from F1 embryos exposed to the high dose, and two control pools isolated from unexposed embryos. For sperm, we sequenced three RRBS libraries prepared from a pool of sperm isolated from F1 males exposed to the high dose, and five RRBS libraries prepared from control pools isolated from unexposed animals.

Repositories


GEO

GSE65784

ArrayExpress

E-GEOD-65784

ENA

SRP053405

BioProject

PRJNA275009

Download


Dataset Statistics

info

Citations per year

Dataset publication