|Number of samples:||12|
|Release date:||Jun 22 2015|
|Last update date:||Oct 10 2018|
|Computational protocol:||Trim Galore!, BSMAP, methylKit, GEO|
|Dataset link||DNA methylation analysis by RRBS in germ cells of mice exposed to Vinclozolin|
Pregnant F0 female mice were exposed to two doses (a low dose VD1=1 mg/kg bw/d and a high dose VD2=100mg/kg bw/d) of Vinclozolin in the drinking water during pregnancy. We isolated primordial germ cells at E13.5 and mature sperm from adult F1 males obtained from control and exposed mothers. For PGCs, we sequenced RRBS libraries prepared from one pool isolated from F1 embryos exposed to the low dose, one pool isolated from F1 embryos exposed to the high dose, and two control pools isolated from unexposed embryos. For sperm, we sequenced three RRBS libraries prepared from a pool of sperm isolated from F1 males exposed to the high dose, and five RRBS libraries prepared from control pools isolated from unexposed animals.
DOI: 10.1371/journal.pone.0124296call_split See protocol