Dataset features

Specifications


Dataset type: Other
Number of samples: 18
Release date: Aug 17 2018
Last update date: Nov 28 2018
Access: Public
Diseases: HIV Infections
Dataset link A Virus-Packageable CRISPR Screen Identifies Host Factors Mediating Interferon Inhibition of HIV

Experimental Protocol


A CRISPR screen targeting Interferon-Stimulated Genes in which enrichment of sgRNA sequences is compared in viral RNA to the library representation in genomic DNA. 2 biological replicates were performed in 3 indpendent cell lines (one wild type THP-1 and two independent ZAP Knockout THP-1 clonal cell lines). Cells were infected following treatment overnight with IFN and viral supernatants and cell pellets collected 3 days post-infection. sgRNA representation in viral RNA is compared to genomic DNA to determine which sgRNAs are over- or under-represented in the viral population.

Repositories


GEO

GSE118631

ENA

SRP158114

BioProject

PRJNA486290

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Contact


Molly Ohainle