|Number of samples:||18|
|Release date:||Aug 17 2018|
|Last update date:||Nov 28 2018|
|Dataset link||A Virus-Packageable CRISPR Screen Identifies Host Factors Mediating Interferon Inhibition of HIV|
A CRISPR screen targeting Interferon-Stimulated Genes in which enrichment of sgRNA sequences is compared in viral RNA to the library representation in genomic DNA. 2 biological replicates were performed in 3 indpendent cell lines (one wild type THP-1 and two independent ZAP Knockout THP-1 clonal cell lines). Cells were infected following treatment overnight with IFN and viral supernatants and cell pellets collected 3 days post-infection. sgRNA representation in viral RNA is compared to genomic DNA to determine which sgRNAs are over- or under-represented in the viral population.