Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Aug 9 2006
Last update date: Mar 16 2012
Access: Public
Dataset link Effect of Cyclic stretching on the gene expression in human spinal ligament cells

Experimental Protocol


Cells were cultured in deformable silicon chamber which were exposed to cyclic stretching (120% extension ratio) for up to 9 hr. After cyclic stretching, total RNA was extracted from each cell monolayer. Target cDNAs transcribed from non-stretched control cells and stretched cells were labeled with Cy3 and Cy5, respectively, and then hybridized with an Agilent Human 1 cDNA Microarray. The microarrays were scanned in both the Cy3 and Cy5 channels with an Affymetrix 428 Array Scanner, and then analyzed using the ImaGene and GeneSight-Lite Software packages.

Repositories


GEO

GSE5464

BioProject

PRJNA95975

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Contact


Ken-Ichi Furukawa