|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Aug 9 2006|
|Last update date:||Mar 16 2012|
|Dataset link||Effect of Cyclic stretching on the gene expression in human spinal ligament cells|
Cells were cultured in deformable silicon chamber which were exposed to cyclic stretching (120% extension ratio) for up to 9 hr. After cyclic stretching, total RNA was extracted from each cell monolayer. Target cDNAs transcribed from non-stretched control cells and stretched cells were labeled with Cy3 and Cy5, respectively, and then hybridized with an Agilent Human 1 cDNA Microarray. The microarrays were scanned in both the Cy3 and Cy5 channels with an Affymetrix 428 Array Scanner, and then analyzed using the ImaGene and GeneSight-Lite Software packages.