Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Dec 11 2009
Last update date: Mar 21 2012
Access: Public
Taxon: Salmonella enterica subsp. enterica serovar Typhi
Dataset link Gene regulation by the RcsCB two-component system and the auxiliary protein TviA in S. Typhi

Experimental Protocol


Gene expression profiles of the S. Typhi wild-type strain, a rcsB mutant, a (delta)tviB-vexE (tviBCDEvexABCDE) mutant, and a (delta)viaB (tviABCDEvexABCDE) mutant (lacking tviA expression) was compared. Strains were cultured in SOB broth (low salt), a condition known to induce expression of TviA and the RcsCB system. Total RNA was extracted from two independent cultures (biological replicates) of each strain. cDNA was differentially labeled with Alexa Fluor 555 and 648, respectively and competitively hybridized on S.Typhimurium arrays (PFGRC, version 4). The submitted samples are biological replicates of a competitive hybridization of cDNA from the wild-type strain (Ty2) and the rcsB mutant (SW237) (GSM395580, GSM395581) or the viaB mutant (STY2) and the tviB-vexE mutant (SW74) (GSM395582, GSM395583), respectively.

Repositories


GEO

GSE15752

ArrayExpress

E-GEOD-15752

BioProject

PRJNA116777

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Contact


Sebastian Winter