Computational protocol: Synthesis and biological characterisation of 18F-SIG343 and 18F-SIG353, novel and high selectivity σ2 radiotracers, for tumour imaging properties

Similar protocols

Protocol publication

[…] The mouse was anaesthetised via inhalant isoflurane (Forthane, Abbott Laboratories, IL, USA) (5% induction, 1% to 3% maintenance in 200 mL/min oxygen). Respiration and heart rates of the animal were monitored (BioVet; m2m Imaging Corp, Cleveland, OH, USA) during the entire scanning period. The core body temperature of the animals was maintained via a temperature-controlled heating pad. The mouse was injected intravenously with 4.6 to 16.7 MBq of 18F-SIG343 or 18F-SIG353, with a constant mass of the unlabelled compounds of 0.06 nmol in 100 μL of saline (n = 3). A 120-min PET scan was performed on an Inveon multimodality positron emission tomography-computed tomography (PET/CT) system (Siemens Medical Solutions, Knoxville, TN, USA) followed by a 10-min CT scan on each subject for anatomical information. Image acquisition commenced simultaneously with radiotracer injection. The data was histogrammed into 25 consecutive frames, and activity volumes were reconstructed with an iterative reconstruction (OSEM/MAP) including attenuation and scatter correction, achieving a reconstructed spatial resolution of 1.5 mm []. Briefly, each individual PET scan was co-registered to its respective CT (automatic and visual quality control) (Anatomist/Brainvisa, version 3.1.4). Regional activity data (Bq/cc) were extracted from nine selected volumes/regions of interest (VOIs/ROIs) where σ receptor distribution was reported and the tail and expressed as percentage of injected dose per cubic centimetres (%ID/cc). […]

Pipeline specifications

Software tools Anatomist, BrainVISA
Application Computerized tomography scan imaging
Organisms Rattus norvegicus, Mus musculus
Diseases Neoplasms