Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 4
Release date: Sep 19 2013
Last update date: May 15 2018
Access: Public
Genes: STAT3, LHX3, ISL1
Dataset link STAT3 promotes motor neuron differentiation by collaborating with motor neuron-specific LIM complex

Experimental Protocol


To explain our experimental scheme briefly, we are interested in finding target sites for the dimer of transcription factors Isl1 and Lhx3. To mimic the biological activity of Isl1/Lhx3 dimer, we made Isl1-Lhx3 fusion and found that Isl1-Lhx3 has a potent biological activity in multiple systems (i.e. generation of ectopic motor neurons). Then we made ES cell line that induces Flag-tagged Isl1-Lhx3 expression upon Dox treatment. These *mouse* ES cells differentiate to motor neurons (iMN-ESCs) when treated with Dox following EB formation. To identify genomic binding sites of Isl1-Lhx3 (Flag-tagged), we performed ChIP with Flag antibody (pull down of Flag-Isl1-Lhx3) in ES cells treated with Dox. ChIP with Flag antibody in ES cells treated with vehicle (no Dox) was done as a negative control in parallel, and sequenced along with +Dox sample. We have done these experiments twice (two sets).

Repositories


GEO

GSE50993

ArrayExpress

E-GEOD-50993

ENA

SRP030018

BioProject

PRJNA219490

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