Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 4
Release date: Aug 1 2018
Last update date: Nov 19 2018
Access: Public
Diseases: Cardiovascular Diseases, Diabetic Neuropathies, Hyperglycemia, Nevus, Sebaceous of Jadassohn
Dataset link Endothelial cells derived from iPSC in response to diabetic medium

Experimental Protocol


Vascular organoids were differentiated from human iPS cells and treated for 3 weeks with a diabetic media containing 75mM Glucose, 1ng/mL TNF-α, 1ng/mL IL6 (DI) or left untreated in 17mM Glucose (NG). Endothelial cells were FACS sorted for CD31 directly into Trizol and stored at -80°C before RNA preparation. The 2 NG and 2 DI are pools of sorted endothelial cells from multiple vascular organoids (>100) from 2 independent differentiations/treatments.

Repositories


GEO

GSE89475

ENA

SRP092491

BioProject

PRJNA352279

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