|Application:||Gene expression microarray analysis|
|Number of samples:||7|
|Release date:||Jan 1 2010|
|Last update date:||Mar 21 2012|
|Dataset link||The Sen1 Helicase Prevents Genome-Wide Spurious Transcription by Controlling Transcription Termination by RNAPII|
 Expression profiling: Genome-wide expression profiling in Sen1 and Nrd1 mutants was performed using Affymetrix tiling microarrays. Termination defects and cryptic transcription were then assessed in pairwise comparisons with wild-type strains that were grown and assayed under the same conditions.  Nucleosome profiling: The supplemental CEL files contain the raw hybridization signals from genome-wide nucleosome occupancy experiments in a SEN1tetO7 and a wild type strain. The .BAR files contain the normalized data for each mutant and wild-type compared to the total genomic DNA signal to identify nucleosome occupancy patterns.