Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 7
Release date: Jan 1 2010
Last update date: Mar 21 2012
Access: Public
Dataset link The Sen1 Helicase Prevents Genome-Wide Spurious Transcription by Controlling Transcription Termination by RNAPII

Experimental Protocol


[1] Expression profiling: Genome-wide expression profiling in Sen1 and Nrd1 mutants was performed using Affymetrix tiling microarrays. Termination defects and cryptic transcription were then assessed in pairwise comparisons with wild-type strains that were grown and assayed under the same conditions. [2] Nucleosome profiling: The supplemental CEL files contain the raw hybridization signals from genome-wide nucleosome occupancy experiments in a SEN1tetO7 and a wild type strain. The .BAR files contain the normalized data for each mutant and wild-type compared to the total genomic DNA signal to identify nucleosome occupancy patterns.

Repositories


GEO

GSE17559

BioProject

PRJNA118659

Download


Contact


Harm Bakel