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Citations per year

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Protocols

ddCt specifications

Information


Unique identifier OMICS_02312
Name ddCt
Alternative name Delta-Delta-Ct
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages R
Computer skills Advanced
Version 1.36.0
Stability Stable
Requirements
methods, BiocGenerics, lattice, RUnit, R(>=2.3.0), xtable, Biobase(>=1.10.0), RColorBrewer(>=0.1-3)
Maintained Yes

Versioning


No version available

Documentation


ddCt citations

 (258)
library_books

Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose utilizing derivatives 8b and 2032

2018
Biotechnol Biofuels
PMCID: 5930841
PMID: 29743953
DOI: 10.1186/s13068-018-1116-x

[…] sate. Cell lysates were diluted 1/10, 1/20, 1/40, 1/80, and 1/160 in the replicates.Plasmid copy numbers (PCN) were determined by ∆∆Ct method [], using the following formulas:Plasmid copy numberPCN=2-ΔΔCt ΔΔCt=ΔCtsample-ΔCtcalibrator plasmid; ΔCtsample=Cttarget1-Ctreference1; ΔCtcalibrator plasmid=Cttarget2-Ctreference2; target1=target region from plasmid; reference1=terminator region ofZ.mobilisc […]

library_books

Pyoverdine, a siderophore from Pseudomonas aeruginosa, translocates into C. elegans, removes iron, and activates a distinct host response

2018
PMCID: 5955448
PMID: 29532717
DOI: 10.1080/21505594.2018.1449508

[…] tantial upregulation after pyoverdine exposure; 2) representation of different gene classes; 3) ability to design 20bp intron-spanning primer with 50% GC content. Fold-changes were calculated using a ΔΔCt method. p-values were calculated by Student's t test. Primer sequences are available upon request. […]

library_books

Promotion of malignant phenotype after disruption of the three dimensional structure of cultured spheroids from colorectal cancer

2018
Oncotarget
PMCID: 5882311
PMID: 29662620
DOI: 10.18632/oncotarget.24641

[…] default settings [].The quantitative PCR reactions were performed with the StepOne Real Time PCR System (Life Technologies, Carlsbad, CA) using Fast SYBR Green Master MIX. The Comparative CT Method (ΔΔCT Method) was applied according to the manufacturer’s instruction. Real Time PCR data were reported as fold-differences relative to the control sample.The sequences of the primers that were used ar […]

library_books

The RNA binding protein Celf1 post transcriptionally regulates p27Kip1 and Dnase2b to control fiber cell nuclear degradation in lens development

2018
PLoS Genet
PMCID: 5889275
PMID: 29565969
DOI: 10.1371/journal.pgen.1007278

[…] yber Green PCR master mix (Invitrogen Life technologies, Carlsbad, CA). Transcript levels were normalized to the housekeeping gene Gapdh. For each sample, differential expression was determined using ΔΔCT method. The following primers were used for qRT-PCR: Celf1-F-5’-ACAGATGAAGCCTGCTGACA-3’ and Celf1-R-5’-CTCTGCTCAAGCCATCAGGT-3’; Dnase2b-F-5’-TGCTCTGGGGAGGACCTTAC-3’ and Dnase2b-R-5’-CCCCTGCGTTCTG […]

call_split

Innate Immune Response and Off Target Mis splicing Are Common Morpholino Induced Side Effects in Xenopus

2018
Dev Cell
PMCID: 5861998
PMID: 29478923
DOI: 10.1016/j.devcel.2018.01.022
call_split See protocol

[…] to ensure only fluorescence was collected from one specific amplicon. B was based on absolute quantification while all other RT-qPCR results were normalized to odc1 and uninjected embryos using the 2−ΔΔCt method (). The threshold cycle (Ct) was derived from the maximum acceleration of SYBR fluorescence (second derivative maximum method). The PCR primers were designed to hybridize at ∼60°C (Tm) and […]

library_books

Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma

2018
Oncotarget
PMCID: 5865674
PMID: 29581848
DOI: 10.18632/oncotarget.24512

[…] Darmstadt, Germany). Quantification of MSX1 was performed as described previously []. For normalization of expression levels we analyzed the transcript of TATA box binding protein (TBP). We used the ddCT-method and the obtained values are indicated in relation to one sample which was set to 1.Quantitative analyses were performed in triplicate. Standard deviations are presented in the figures as e […]


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