ddCt protocols

View ddCt computational protocol

ddCt statistics

To access cutting-edge analytics on consensus tools, life science contexts and associated fields, you will need to subscribe to our premium service.

Subscribe
info

Citations per year

Citations chart
info

Popular tool citations

chevron_left Data analysis chevron_right
Popular tools chart
info

Tool usage distribution map

Tool usage distribution map
info

Associated diseases

Associated diseases

ddCt specifications

Information


Unique identifier OMICS_02312
Name ddCt
Alternative name Delta-Delta-Ct
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages R
Computer skills Advanced
Version 1.36.0
Stability Stable
Requirements
methods, BiocGenerics, lattice, RUnit, R(>=2.3.0), xtable, Biobase(>=1.10.0), RColorBrewer(>=0.1-3)
Maintained Yes

Versioning


Add your version

Documentation


ddCt in pipeline

2015
PMCID: 4639806
PMID: 26506265
DOI: 10.1038/ncomms9466

[…] ranged from 100–250 bp. suitability of qpcr primer was analysed by evaluation of melting curves and by determination of the efficiency via a standard curve. for quantitative expression analysis, the delta delta ct method was applied to determine the relative quantity of target sequence using a reference sample (control) and an endogenous control target sequence. rpl27 was used as endogenous […]


To access a full list of citations, you will need to upgrade to our premium service.

ddCt in publications

 (1682)
PMCID: 5955967
PMID: 29769603
DOI: 10.1038/s41598-018-26051-x

[…] and the efficiency of the rrna 18s gen (endogenous control) amplification were approximately equal, so the relative gene expression of the above mentioned genes could be obtained using the delta-delta ct (δδct) method normalizing for rrna 18s. multiple protein sequence alignment was conducted with muscle program, and then curated by gblocks to select conserved blocks of amino acids. […]

PMCID: 5935742
PMID: 29728632
DOI: 10.1038/s41598-018-25395-8

[…] and reverse primer sequences used to conduct gene expression analysis. ribosomal rna (rrna) 18 s was used as the housekeeping gene for normalization. mrna transcript levels were calculated using the delta delta ct (∆∆ct) method, normalized to rrna 18 s expression as follows: x = 2 − ∆∆ct, in which ∆∆ct = (ctexp − ct18s) − (ctctl − ct18s) and x = relative transcript; ctctl = ct of control group., […]

PMCID: 5935693
PMID: 29728608
DOI: 10.1038/s41598-018-25008-4

[…] expressing all other tdp-43 variants were transfected with one concentration, 0.5 ug. transcript levels of tdp-43, cftr +9, and cftr −9 were calculated using quantitative real-time pcr, using the delta delta ct method with hprt as a loading control. for each sample, normalized splicing index was calculated as: normalized splicing index = (cftr +9)/((cftr −9)(tdp-43)). the normalized splicing […]

PMCID: 5945818
PMID: 29780306
DOI: 10.3389/fncel.2018.00120

[…] states) and a cfx connect optics module (bio-rad, hercules, ca, united states). comparison of gene expression among samples was conducted using the pfaffl method which is a modified version of the delta-delta ct equation that takes into account the efficiency of each primer set (). gene expression was normalized to a housekeeping gene (rpl19), which has been used previously for qpcr […]

PMCID: 5932055
PMID: 29720621
DOI: 10.1038/s41598-018-25358-z

[…] 5′-ggtgtccaagaacgtgttgtt-3′; mbp: forward, 5′-gcaaacgctttcgatgggttg-3′; reverse, 5′-acacagtgagatagacgccag-3′; β-actin: forward, 5′-agagggaaatcgtgcgrgac-3′; reverse, 5′-caatagtgacctggccgt-3′. the delta-delta ct method analyzed mrna expression, and the relative fold was normalized to β-actin., firstly, non-adherent mononuclear cells were harvested from mice bone marrow and cultured in imdm […]


To access a full list of publications, you will need to upgrade to our premium service.

ddCt reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review ddCt