ddCt protocols

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ddCt specifications


Unique identifier OMICS_02312
Name ddCt
Alternative name Delta-Delta-Ct
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages R
Computer skills Advanced
Version 1.36.0
Stability Stable
methods, BiocGenerics, lattice, RUnit, R(>=2.3.0), xtable, Biobase(>=1.10.0), RColorBrewer(>=0.1-3)
Maintained Yes


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ddCt in pipeline

PMCID: 4639806
PMID: 26506265
DOI: 10.1038/ncomms9466

[…] ranged from 100–250 bp. suitability of qpcr primer was analysed by evaluation of melting curves and by determination of the efficiency via a standard curve. for quantitative expression analysis, the delta delta ct method was applied to determine the relative quantity of target sequence using a reference sample (control) and an endogenous control target sequence. rpl27 was used as endogenous […]

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ddCt in publications

PMCID: 5955967
PMID: 29769603
DOI: 10.1038/s41598-018-26051-x

[…] and the efficiency of the rrna 18s gen (endogenous control) amplification were approximately equal, so the relative gene expression of the above mentioned genes could be obtained using the delta-delta ct (δδct) method normalizing for rrna 18s. multiple protein sequence alignment was conducted with muscle program, and then curated by gblocks to select conserved blocks of amino acids. […]

PMCID: 5935742
PMID: 29728632
DOI: 10.1038/s41598-018-25395-8

[…] and reverse primer sequences used to conduct gene expression analysis. ribosomal rna (rrna) 18 s was used as the housekeeping gene for normalization. mrna transcript levels were calculated using the delta delta ct (∆∆ct) method, normalized to rrna 18 s expression as follows: x = 2 − ∆∆ct, in which ∆∆ct = (ctexp − ct18s) − (ctctl − ct18s) and x = relative transcript; ctctl = ct of control group., […]

PMCID: 5935693
PMID: 29728608
DOI: 10.1038/s41598-018-25008-4

[…] expressing all other tdp-43 variants were transfected with one concentration, 0.5 ug. transcript levels of tdp-43, cftr +9, and cftr −9 were calculated using quantitative real-time pcr, using the delta delta ct method with hprt as a loading control. for each sample, normalized splicing index was calculated as: normalized splicing index = (cftr +9)/((cftr −9)(tdp-43)). the normalized splicing […]

PMCID: 5945818
PMID: 29780306
DOI: 10.3389/fncel.2018.00120

[…] states) and a cfx connect optics module (bio-rad, hercules, ca, united states). comparison of gene expression among samples was conducted using the pfaffl method which is a modified version of the delta-delta ct equation that takes into account the efficiency of each primer set (). gene expression was normalized to a housekeeping gene (rpl19), which has been used previously for qpcr […]

PMCID: 5932055
PMID: 29720621
DOI: 10.1038/s41598-018-25358-z

[…] 5′-ggtgtccaagaacgtgttgtt-3′; mbp: forward, 5′-gcaaacgctttcgatgggttg-3′; reverse, 5′-acacagtgagatagacgccag-3′; β-actin: forward, 5′-agagggaaatcgtgcgrgac-3′; reverse, 5′-caatagtgacctggccgt-3′. the delta-delta ct method analyzed mrna expression, and the relative fold was normalized to β-actin., firstly, non-adherent mononuclear cells were harvested from mice bone marrow and cultured in imdm […]

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