|Application:||Gene expression microarray analysis|
|Number of samples:||72|
|Release date:||Feb 1 2008|
|Last update date:||Mar 19 2012|
|Genes:||ABL1, BCR, STAT5A, TGFB1, CISH, SOCS2, SOCS3, PIM1, DUSP6, TNFAIP3|
|Dataset link||BCR/ABL1-Dependent Transcriptional Response Reveals Enrichment for Genes Involved in Negative Feedback Regulation|
Five Philadelphia-positive (Ph+) and five Philadelphia-negative (Ph-) cell lines were cultured in the absence or presence of 1 microM imatinib mesylate for 3 and 12 hours. Total RNA was extracted from untreated and treated cell cultures at both time points, resulting in 4 samples per cell line. RNA extraction, labeling, hybridization, washing, scanning, and feature analysis were performed as described (Håkansson et al., 2008, Genes Chromosomes Cancer). Dye-swap labeling was performed in 32 out of 40 samples. In total, 72 slides were hybridized and scanned. The transcriptional response was studied using the mean value of the 3 and 12 hour microarray measurements.