Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 72
Release date: Feb 1 2008
Last update date: Mar 19 2012
Access: Public
Diseases: Leukemia, Neoplasms
Genes: ABL1, BCR, STAT5A, TGFB1, CISH, SOCS2, SOCS3, PIM1, DUSP6, TNFAIP3
Dataset link BCR/ABL1-Dependent Transcriptional Response Reveals Enrichment for Genes Involved in Negative Feedback Regulation

Experimental Protocol


Five Philadelphia-positive (Ph+) and five Philadelphia-negative (Ph-) cell lines were cultured in the absence or presence of 1 microM imatinib mesylate for 3 and 12 hours. Total RNA was extracted from untreated and treated cell cultures at both time points, resulting in 4 samples per cell line. RNA extraction, labeling, hybridization, washing, scanning, and feature analysis were performed as described (Håkansson et al., 2008, Genes Chromosomes Cancer). Dye-swap labeling was performed in 32 out of 40 samples. In total, 72 slides were hybridized and scanned. The transcriptional response was studied using the mean value of the 3 and 12 hour microarray measurements.

Repositories


GEO

GSE10283

BioProject

PRJNA108487

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Contact


Petra Johnels
Petra Håkansson
Petra HÃ¥kansson