Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Feb 20 2009
Last update date: Jan 18 2013
Access: Public
Diseases: Neoplasms, Prostatic Neoplasms
Dataset link Gene Expression in Castration-Resistant C4-2 Cells after AR Knockdown

Experimental Protocol


C4-2 human prostate cancer cells stably expressing a tetracycline-inducible AR-targeted short hairpin RNA (shRNA) or scrambled shRNA were generated. These two cell lines were incubated in the absence of androgens with or without doxycycline hyclase (DOX). Comparison analysis of the gene expression profiles of C4-2 cells stably expressing AR shRNA + DOX and control cells (AR shRNA - DOX and scrambled shRNA ┬▒ DOX) was conducted to identify differentially regulated genes due to AR knockdown after normalization and data filtering. Genes were considered to be significantly different if the expression in the induced AR shRNA + DOX cells was at least 1.7-fold greater or 1.7-fold less than that seen in the control cells, with P< 0.05.

Repositories


GEO

GSE13332

ArrayExpress

E-GEOD-13332

BioProject

PRJNA109849

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Contact


Helen Cheng
Helen SW Cheng