|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Feb 20 2009|
|Last update date:||Jan 18 2013|
|Diseases:||Neoplasms, Prostatic Neoplasms|
|Dataset link||Gene Expression in Castration-Resistant C4-2 Cells after AR Knockdown|
C4-2 human prostate cancer cells stably expressing a tetracycline-inducible AR-targeted short hairpin RNA (shRNA) or scrambled shRNA were generated. These two cell lines were incubated in the absence of androgens with or without doxycycline hyclase (DOX). Comparison analysis of the gene expression profiles of C4-2 cells stably expressing AR shRNA + DOX and control cells (AR shRNA - DOX and scrambled shRNA ± DOX) was conducted to identify differentially regulated genes due to AR knockdown after normalization and data filtering. Genes were considered to be significantly different if the expression in the induced AR shRNA + DOX cells was at least 1.7-fold greater or 1.7-fold less than that seen in the control cells, with P< 0.05.
Helen SW Cheng