Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 4
Release date: Dec 28 2017
Last update date: Oct 11 2018
Access: Public
Diseases: Persistent Hyperplastic Primary Vitreous
Dataset link Single-cell RNA sequencing of vascularized brain organoids

Experimental Protocol


VBOrs were generated by two methods distinct in the step of vascular progenitor induction: method 1 (group BMPi), EBs were treated with bFGF, VEGF and SB431542 for two days; method 2 (group BMPa), EBs were treated with bFGF, VEGF and BMP4 for two days. Single-cell suspensions from two groups of VBOrs (BMPa and BMPi) were prepared by 20-min digestion in 0.25 mg/ml trypsin. About 10,000-12,000 cells for each sample (2 independent repeats) were loaded for soating, barcoding and library construction. Sequencing was performed with HiSeq X.

Repositories


GEO

GSE108571

ENA

SRP127624

BioProject

PRJNA427709

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Contact


Xiang-Chun Ju