|Number of samples:||4|
|Release date:||Dec 28 2017|
|Last update date:||Oct 11 2018|
|Diseases:||Persistent Hyperplastic Primary Vitreous|
|Dataset link||Single-cell RNA sequencing of vascularized brain organoids|
VBOrs were generated by two methods distinct in the step of vascular progenitor induction: method 1 (group BMPi), EBs were treated with bFGF, VEGF and SB431542 for two days; method 2 (group BMPa), EBs were treated with bFGF, VEGF and BMP4 for two days. Single-cell suspensions from two groups of VBOrs (BMPa and BMPi) were prepared by 20-min digestion in 0.25 mg/ml trypsin. About 10,000-12,000 cells for each sample (2 independent repeats) were loaded for soating, barcoding and library construction. Sequencing was performed with HiSeq X.