|Application:||Gene expression microarray analysis|
|Number of samples:||24|
|Release date:||May 30 2016|
|Last update date:||Dec 11 2017|
|Dataset link||Comparison of the in vivo transcriptome of the myometrium to the transcriptome of myometrial explants, primary cultured myometrial cells and the hTert myometrial cell line|
Biopsies obtained from non-laboring women at elective Caesarean section at term were divided into 3: (i) dissected and immediately snap-frozen (t=0), (ii) dissected into 3x3x3mm3myometrial explants and (iii) processed for primary cell culture. Explants, primary cells at passage 4 (the typical passage our group uses for experiments) and hTERT cells were cultured for a period of 30 hours without treatment. Total RNA was extracted and microarray analysis performed. 6 replicates were used for this study.