Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: May 30 2016
Last update date: Dec 11 2017
Access: Public
Chemicals: Progesterone
Genes: FHR1
Dataset link Comparison of the in vivo transcriptome of the myometrium to the transcriptome of myometrial explants, primary cultured myometrial cells and the hTert myometrial cell line

Experimental Protocol


Biopsies obtained from non-laboring women at elective Caesarean section at term were divided into 3: (i) dissected and immediately snap-frozen (t=0), (ii) dissected into 3x3x3mm3myometrial explants and (iii) processed for primary cell culture. Explants, primary cells at passage 4 (the typical passage our group uses for experiments) and hTERT cells were cultured for a period of 30 hours without treatment. Total RNA was extracted and microarray analysis performed. 6 replicates were used for this study.

Repositories


GEO

GSE77830

ArrayExpress

E-GEOD-77830

BioProject

PRJNA311707

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