Computational protocol: Analysis of Kinetoplast DNA from Mexican Isolates of Leishmania (L.) mexicana

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Protocol publication

[…] The sequences were edited with the DNAMAN, Chromas version 2.0, and Seaview software []. Multiple sequences were aligned using Clustal-X Ver. 1.83 []. An unrooted phylogenetic tree was constructed using the neighbor-joining method [] with the Clustal-X program. Evolutionary distances were calculated using Kimura's two-parameter method [], with MEGA (Molecular Evolutionary Genetics Analysis), Version 3.1. []. A total of 1000 bootstrapping replicates were used for the neighbor-joining analysis to obtain relative support for internal nodes. The kDNA sequence data were compared with the sequences of the other members of the L. mexicana complex previously published in GenBank. […]

Pipeline specifications

Software tools DNAMAN, SeaView, Clustal W, MEGA
Application Phylogenetics
Organisms Leishmania mexicana, Homo sapiens
Diseases Leishmaniasis, Sprains and Strains