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DeadEasy Larval Glia specifications


Unique identifier OMICS_27813
Name DeadEasy Larval Glia
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Java
Computer skills Medium
Stability Stable
Maintained Yes


  • Invertebrates
    • Drosophila melanogaster




No version available


  • person_outline Alicia Hidalgo <>

Publication for DeadEasy Larval Glia

DeadEasy Larval Glia citations


Three tier regulation of cell number plasticity by neurotrophins and Tolls in Drosophila

PMCID: 5412559
PMID: 28373203
DOI: 10.1083/jcb.201607098

[…] images, and adjust image size to 300 dpi. illustrator (adobe) was used to compile figure plates. (b) for quantitative data (e.g., number of dcp1+, eve+, or yfp+ cells), we used the imagej plugins deadeasy larval glia (which counts nuclear stains) and deadeasy caspase for larvae (for apoptotic cells) as previously described and validated (, ; ). deadeasy analyzes the entire stacks of images […]


Molecular mechanism of central nervous system repair by the Drosophila NG2 homologue kon tiki

PMCID: 5004445
PMID: 27551055
DOI: 10.1083/jcb.201603054

[…] kon mutants are lethal in the embryo, we tested the effects of kon knockdown using rnai. the total number of repo+ glial cells in third-instar larval vncs were counted in vivo automatically using deadeasy larval glia software (). kon knockdown in all neurons (elavgal4>uaskonrnai106680) or only the pros+ ng (alrmgal4>konrnai106680), did not affect total glial cell number (). however, kon […]


An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

PMCID: 3641668
PMID: 23567253
DOI: 10.3791/50306

[…] that upon injury some of the dying cells in the cortex are neurons. np, neuropile; cx, cortex., figure 4. automatic counting of glial cells and apoptotic cells using deadeasy.(a) example of using 'deadeasy larval glia' to count repo positive glial cells. left: vnc ventral halves stained with anti-repo antibodies; middle: output from deadeasy larval glia showing cells identified by the plug-in; […]


The Glial Regenerative Response to Central Nervous System Injury Is Enabled by Pros Notch and Pros NFκB Feedback

PMCID: 3166069
PMID: 21912512
DOI: 10.1371/journal.pbio.1001133

[…] the videos were arranged using imagej and adobe photoshop., to count automatically the number of all larval glia stained with anti-repo and acquired as confocal microscopy images, we purposely wrote deadeasy larval glia software in java as an imagej plug-in. confocal serial sections were obtained with the biorad radiance 2000 confocal microscope as images of x,y = 0.5665 µm/pixel and z = 1 […]

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DeadEasy Larval Glia institution(s)
Neurodevelopment Group, School of Biosciences, University of Birmingham, Birmingham, UK

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