Deamidation site detection software tools | Post-translational modification data analysis
Protein deamidation is a post-translational modification in which the side chain amide group of a glutamine or asparagine residue is transformed into an acidic carboxylate group. Non-enzymatic deamidation of asparagine is faster than of glutamine and hence presents higher physiological significance, being involved in processes such as apoptosis, brain development and aging. Deamidation often, but not always, leads to loss of protein function.
An algorithm for the prediction of spontaneous non-enzymatic deamidation of internal asparagine residues in proteins. Asparagine deamidation is modulated by protein local sequence, secondary structure and hydrogen bonding. However, NGOME makes use of sequence-based predictions of secondary structure and intrinsic disorder and does not require a protein structure. NGOME may help the user identify deamidation-prone asparagine residues that may act as a molecular clock for the regulation of protein function and turnover.