|Application:||Gene expression microarray analysis|
|Number of samples:||3|
|Release date:||Sep 18 2010|
|Last update date:||Oct 29 2018|
|Genes:||KMT5A, IL17RB, DTL|
|Dataset link||Stable Set8 effect on gene expression in U2OS cells|
We used microarrays to detail the effect of the expression of stable form of Set8b H4K20 mono-methyl transferase (Set8b_deltaPIP2) on gene expression Human osteosarcoma-derived human cells were transduced with retroviruses encoding either wt-Set8b or a mutant of Setb8 which is resistant to degradation via CRL4-Cdt2 ubiquitin ligase complex (Set8b_deltaPIP2) or with an a control pMSCV empty virus. 5 days after transduction, cells were harvested and the RNA was extracted by Trizol (Invitrogen) and hybridized to the affymetrix chips array.