Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 3
Release date: Sep 18 2010
Last update date: Oct 29 2018
Access: Public
Genes: KMT5A, IL17RB, DTL
Dataset link Stable Set8 effect on gene expression in U2OS cells

Experimental Protocol


We used microarrays to detail the effect of the expression of stable form of Set8b H4K20 mono-methyl transferase (Set8b_deltaPIP2) on gene expression Human osteosarcoma-derived human cells were transduced with retroviruses encoding either wt-Set8b or a mutant of Setb8 which is resistant to degradation via CRL4-Cdt2 ubiquitin ligase complex (Set8b_deltaPIP2) or with an a control pMSCV empty virus. 5 days after transduction, cells were harvested and the RNA was extracted by Trizol (Invitrogen) and hybridized to the affymetrix chips array.

Repositories


GEO

GSE24170

ArrayExpress

E-GEOD-24170

BioProject

PRJNA130091

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Contact


Anindya Dutta