Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 27
Release date: Jun 12 2017
Last update date: Nov 29 2018
Access: Public
Genes: INTS12, EIF2AK3
Taxon: Homo sapiens
Dataset link Integrator complex subunit 12 is a key regulator of human protein synthesis pathways

Experimental Protocol


INTS12 depletion was induced in human bronchial epithelial cells from both donors. RNAseq gene expression profiling was performed 48h and 120h since the initiation of knockdown in the first and second donor respectively. Silencing was achieved with two independent D-siRNAs and experiments included scambled D-siRNA transfected and un-transfected cells. Experiments were performed in three biological replicates. Genome-wide INTS12 binding sites were inferred by INTS12 ChIPseq in two donor cells. ChIPseq experiment included input control pooled from both sequenced donors.

Repositories


GEO

GSE80386

ENA

SRP073445

BioProject

PRJNA318778

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Contact


Alexander Kheirallah

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