Mutagenesis provides insight into proteins, but only recently have assays that couple genotype to phenotype been used to assess the activities of as many as 1 million mutant versions of a protein in a single experiment. This approach-'deep mutational scanning'-yields large-scale data sets that can reveal intrinsic protein properties, protein behavior within cells and the consequences of human genetic variation. Deep mutational scanning is transforming the study of proteins, but many challenges must be tackled for it to fulfill its promise. Source text: Fowler and Fields, 2014.
dms_tools
dms_tools
A software package to infer the impacts of mutations from deep mutational scanning data…
A software package to infer the impacts of mutations from deep mutational scanning data using a likelihood-based treatment of the mutation counts. Using dms_tools, one can infer the preference of each site for each amino acid given a single…
Enrich
Enrich
A tool for analyzing such deep mutational scanning data. Enrich identifies all unique…
A tool for analyzing such deep mutational scanning data. Enrich identifies all unique variants (mutants) of a protein in high-throughput sequencing datasets and can correct for sequencing errors using overlapping paired-end reads. Enrich uses the…