Definetherain statistics

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Definetherain specifications


Unique identifier OMICS_03945
Name Definetherain
Software type Application/Script
Interface Web user interface
Restrictions to use None
Biological technology Bio-Rad Laboratories
Programming languages Javascript
License MIT License
Computer skills Basic
Stability Stable
Source code URL
Maintained Yes


  • person_outline John Frater <>

Publication for Definetherain

Definetherain in publications

PMCID: 5789538
PMID: 29378600
DOI: 10.1186/s12977-018-0399-0

[…] et al. defines the median and variance of the negative and positive clouds to assess the statistical likelihood that outliers should be included in either cloud (p < 0.1). jones et al. developed “definetherain” that uses negative and positive control samples to identify the two clouds. subsequently, the mean fluorescence minus or plus three times its standard deviation is used as thresholds […]

PMCID: 5167268
PMID: 27992475
DOI: 10.1371/journal.pone.0168179

[…] (vii) irregular droplet size []., software has been developed for better evaluation of ddpcrs with droplet rain, either to improve the threshold setting, such as ddpcrquant [], or software such as definetherain [] that excludes intermediate droplets for analysis. if the rain is caused by biased amplification efficiency, another strategy to improve accuracy and avoid incorrect thresholds […]

PMCID: 5154634
PMID: 27990345
DOI: 10.1016/j.bdq.2016.05.002

[…] setting and to try and standardise the data analysis method, three recent publications have described methods to aid operators to set the thresholds appropriately. one describes a javascript code ‘definetherain’ where the threshold is determined by the k-nearest neighbour algorithm of a control sample . the second method uses a manual global threshold (mtg) approach that is based […]

PMCID: 4827695
PMID: 27077048
DOI: 10.1016/j.bdq.2015.12.003

[…] of rain can hinder analysis and the correct setting of a threshold. several approaches exist to minimize the effects of rain on quantitative results , . unfortunately, the existing algorithms like ‘definetherain’ consider only the fam channel of the qx ddpcr system, while disregarding the hex/vic channel., an important task of official food and feed control in the european union (eu) […]

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Definetherain institution(s)
Peter Medawar Building for Pathogen Research, Nuffield Department of Clinical Medicine, Oxford, UK; IUH, Hôpital St. Louis, Paris, France; Institute for Emerging Infections, The Oxford Martin School, Oxford, UK; Oxford National Institute of Health Research Biomedical Research Centre, Oxford, UK

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