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ddCt

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The ddCt Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.

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ddCt classification

ddCt specifications

Unique identifier:
OMICS_02312
Software type:
Package/Module
Restrictions to use:
None
Programming languages:
R
Stability:
Stable
Name:
Delta-Delta-Ct
Interface:
Command line interface
Operating system:
Unix/Linux, Mac OS, Windows
Computer skills:
Advanced
Maintained:
Yes

ddCt distribution

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ddCt support

Documentation

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